INTRODUCTION: Food insecurity and limited healthful food access are critical public health issues in the United States (U.S), with unequal distribution across regions. This report tracked the prevalence of food insecurity, healthful food access, and the food environment, as cultural experiences according to the American Nations Model. METHODS: Data from the County Health Rankings & Roadmaps program on food insecurity, insufficient healthy food access, and the food environment index were matched at the zip-code level with the American Nations dataset from the Nationhood Lab. Percentages for all three food indicators were estimated based on the population of each American Nation region. RESULTS: Results show significant disparities across regional cultures. The First Nation, heavily populated by American Indian and Alaska Native communities, reports the highest prevalence of food insecurity (17 %), lowest healthful food access (21 %) and food environment index (4.6 out of 10). New France, Deep South, Great Polynesia, Greater Appalachia, El Norte and Far West, areas with higher minority populations, also show elevated food insecurity rates (range, 11-14 %) and reduced healthful food access (5-9 %). Regions with more favorable metrics across all three indicators include the Spanish Caribbean, Midlands, Yankeedom, Left Coast, Tidewater, and the New Netherlands. CONCLUSIONS: Disparities in food insecurity, healthful food access, and food environments appear to stem from geographical diversity and cultural history, underscoring the need to recognize and address cultural differences among the American Nations. This insight can inform policy and practices aimed at achieving food security and health equity across the country.